Reporter

Part:BBa_K400628

Designed by: Seobeom Heo   Group: iGEM10_Korea_U_Seoul   (2010-10-19)


mAAA (EcoRI site mutated Aryl Acyl-Amidase)

Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is reversible. When a p-acetaminophenol was reacted with AAA, the product gave a purple-red color. As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it was grown on solid media enriched with p-acetaminophenol.

Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter. In our project, we put it downstream of cadmium-dependent promoter to detect any cadmium present in a solution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 328
    Illegal NgoMIV site found at 1365
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None