Part:BBa_K4003002
T7 promoter
profile
Name: T7 promoter
Base Pairs: 19bp
Origin: T7 phage, genome
Properties: A promoter for initiation of the transcription.
Usage and Biology
The promoter can react specifically to T7 RNA polymerase and is a sequence that starts gene transcription of T7 phage. It is recognized for binding and initiation of the transcription. Placed before a gene, it promotes its transcription.
Experimental approach
Production, purification, and SDS-PAGE analysis of recombinant PPM1A
Next, we screened the PPM1A activator in Lab in-house compound library by phosphatase enzyme activity assay. The effect of compounds 5 (0.01, 0.1, 1, 5, 10, 20, 40, 100 μM) on the PPM1A was detected by phosphatase activity assay with pNPP as the substrate. All data were presented as mean ± S.E.M (*P<0.05, **P< 0.01, ***P< 0.001).
As indicated in Figure 6, among the compounds, Compound 5 was finally selected for its highest enzymatic activity against PPM1A. Besides, it could also tell that compound 5 dose-dependently enhanced PPM1A enzyme activity. These results thus implied that compound 5 was a PPM1A enzymatic activator.
Reference:
1) Cohen, P.T.W. Overview of protein serine/threonine phosphatases. Protein Phosphatases 2004.
2) Smith SR, Schaaf K, Rajabalee N, et al. The phosphatase PPM1A controls monocyte-to-macrophage differentiation. Sci Rep. 2018; 8(1):902.
3) Hansen DV, Hanson J E, Sheng M. Microglia in alzheimer's disease. J Cell Biol 2017; 217:459-472.
4) Paolicelli, R. C., Jawaid, A., Henstridge, C. M., Valeri, A., Merlini, M., Robinson, J. L., Lee, E. B., Rose, J., Appel, S., Lee, V. M. Y., Trojanowski, J. Q., Spires-Jones, T., Schulz, P. E. & Rajendran, L. 2017. Tdp-43 Depletion In Microglia Promotes Amyloid Clearance But Also Induces Synapse Loss. Neuron, 95, 297-308.E6. doi: 10.1016/j.neuron.2017.05.037
5) Wilson, R. S., Yu L Fau – Trojanowski, J. Q., Trojanowski Jq Fau – Chen, E.-Y., Chen Ey Fau – Boyle, P. A., Boyle Pa Fau – Bennett, D. A., Bennett Da Fau – Schneider, J. A. & Schneider, J. A. 2013. Tdp-43 Pathology, Cognitive Decline, And Dementia In Old Age. JAMA Neurol, 70, 1418-1424. doi: 10.1001/jamaneurol.2013.3961.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |