Regulatory

Part:BBa_K4000003

Designed by: Ke ZHU   Group: iGEM21_Fujian_United   (2021-10-12)


HXT7p

Profile

Name: HXT7p

Base Pairs: 587bp

Origin: Saccharomyces cerevisiae, genome

Properties: Inducible promoter regulated by glucose

Usage and Biology

HXT7 promoter is one of the high-affinity hexose transporter, it is sufficient for complementary expression of invertase to restore the growth of Saccharomyces cerevisiae in raffinose medium. HXT7 promoter could be used for heterologous protein expression in S. cerevisiae.

Figure1. Principle diagram of GA..

Experimental approach

1. Fragments PCR products Electrophoresis

Figure 2. Gel electrophoresis of amplified fragments..

The basic part of the plasmid, HXT7 promoters, was amplified successfully firstly.

References

1. Görgens J F, Bressler D C, van Rensburg E. Engineering Saccharomyces cerevisiae for direct conversion of raw, uncooked or granular starch to ethanol[J]. Critical reviews in biotechnology, 2015, 35(3): 369-391. =

2. Van Zyl W H, Bloom M, Viktor M J. Engineering yeasts for raw starch conversion[J]. Applied microbiology and biotechnology, 2012, 95(6): 1377-1388.

3. Maury J, Kannan S, Jensen N B, et al. Glucose-dependent promoters for dynamic regulation of metabolic pathways[J]. Frontiers in bioengineering and biotechnology, 2018, 6: 63.

4. Weber E, Engler C, Gruetzner R, et al. A modular cloning system for standardized assembly of multigene constructs[J]. PloS one, 2011, 6(2): e16765. =

5. Pollak B, Cerda A, Delmans M, et al. Loop assembly: a simple and open system for recursive fabrication of DNA circuits[J]. New Phytologist, 2019, 222(1): 628-640.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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