Composite

Part:BBa_K400000

Designed by: Jonghwa Kim   Group: iGEM10_Korea_U_Seoul   (2010-10-21)

composite heavy metal-detector(Zn, Cd, As)

It is a composite part of heavy metal-detecting promoters and reporter genes.

PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)----

It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a capsule in order to be customized. [http://2010.igem.org/Team:Korea_U_Seoul/Project#Result]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2105
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 3731
    Illegal AgeI site found at 4516
    Illegal AgeI site found at 4628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1144
    Illegal BsaI.rc site found at 5410


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Categories
Parameters
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