Coding

Part:BBa_K3998001

Designed by: Xiaoyan Wei   Group: iGEM21_Shanghai_HS_United   (2021-10-12)


chi

Profile

Name: chi

Base Pairs: 510bp

Origin: From Genebank

Properties: A protein used to improve the degradation of flavonoids

Usage and Biology

In this project, we put the chi gene into E. coli to produce a strain secreting chi enzyme efficiently. Protein of Chi convert dihydroflavone into chalcone to improve the degradation of flavonoids, further produce DAT and stimulate the immune system of the human body, so as to achieve anti-inflammatory, antibacterial, anti-cancer and other purposes, at the same time to help reducing clinical treatment costs.

Figure1 Principles of the degradation of intestinal bacteria flavonoids: Flavonoids - > dihydroflavones - > charone - > dihydrocarboncarbonone..
Figure2 Schematic map of pET 28a and chi expression plasmids..

References

1. Gaohua Yang, Sen Hong, Pengjie Yang, et al. Discovery of an ene-reductase for initiating flavone and flavonol catabolism in gut bacteria, Nature communcations (2021).

2. 黄瑶. 黄酮类物质改善认知功能障碍作用机制的研究进展[J]. 养生大世界 2021年4期, 241-242页, 2021.

3. Thilakarathna S H, Rupasinghe HP. Flavonoids Bioavailability and attempts for bioavailability enhancement[J].Nutrients, 2013, 5(9):3367-3387

4. Ravishankar D, Rajora A K, Greco F, et al. Flavonoids as prospective compounds for anti-cancer therapy[J].Int J Bio- chem Cell Biol, 2013, 45(12):2821-2831

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


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