Part:BBa_K3985005

eGFP codon optimized for B. subtilis

Enhanced green fluorescent protein (eGFP) is derived from the jellyfish Aequorea victoria and is one of the best-studied proteins in biochemistry. eGFP has an excitation maximum at 488 nm and an emission maximum at 507 nm. It is used to monitor gene expression efficiently in a variety of cells. [1]

In our genetic circuit eGFP is unter the control of the inducable promoter P_PA1897 or P_Lux. If the acyl homoserine lactone 3OC₁₂-HSL is present the expression of the gene encoding eGFP is induced. By that, we test the function of our designed genetic construct.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Guohong Zhang, Vanessa Gurtu, Steven R. Kain. 1996. An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells. Biochemical and Biophysical Research Communications. 227: 707– 711. doi: 10.1006/bbrc.1996.1573.