Coding

Part:BBa_K3985003

Designed by: Antonia Schetle, Melina Baur   Group: iGEM21_TU_Darmstadt   (2021-10-15)


QscR codon optimized for B. subtilis

QscR is a quorum sensing controlling repressor and originates from P. aeruginosa. QscR regulates the gene expression of quorum sensing genes. It binds the acyl homoserine lactone N-(3-oxododecanoyl)-homoserine lactone (3OC12-HSL). 3OC12-HSL is an autoinducer by which gene expression of P. aeruginosa can be regulated. By forming a complex with 3OC12-HSL, QscR induces PPA1897.[1]

In our designed genetic circuit, the gene encoding the transcription factor QscR is controlled by a constitutive promoter originating from B. subtilis. The allosteric transcription factor binds the autoinducer 3OC12-HSL and thus, its presence can be detected through eGFP expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None