Coding

Part:BBa_K3985001

Designed by: Melina Baur, Antonia Schetle   Group: iGEM21_TU_Darmstadt   (2021-10-15)


LasR codon optimized for B. subtilis

LasR is an allosteric trancription factor which originates from P. aeruginosa and regulates the gene expression of quorum-sensing genes. It binds the acyl homoserine lactone N-(3-oxododecanoyl)-homoserine lactone (3OC12-HSL). 3OC12-HSL is an autoinducer by which gene expression in P. aeruginosa is regulated. By forming a complex with 3OC12-HSL, LasR induces the promoter PLux. [1]

In our designed genetic circuit, the gene encoding the transcription factor LasR is controlled by a constitutive promoter originating from B. subtilis. The allosteric transcription factor binds the autoinducer 3OC12-HSL and thus its presence can be detected through eGFP expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 35


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Categories
Parameters
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