Tag

Part:BBa_K3982003

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)


6x Histidine Tag

6x Histidine Tag is used in Project CODE M by Team IISER_Berhampur 2021

Usage and Biology

6x Histidine Tag is to tagged to Cas14a1 gene BBa_K3982001 along with TEV protease BBa_K3982002 for easy purification of the Cas14a1 protein by using affinity chromatography. This is a very widely used tag for various proteins.

Reference

L. B. Harrington et al., Science, 10.1126/science.aav4294 (2018).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None