Generator

Part:BBa_K398026

Designed by: Kira Schipper   Group: iGEM10_TU_Delft   (2010-10-07)


J23100_J61101_ADH_J61107_ALDH

ADH, an alcohol dehydrogenase isolated from Bacillus thermoleovorans B23, is capable of converting n-alkanols into the corresponding n-alkanal, the second step in the biodegradation of alkanes. From this same microorganism we will also be using ALDH, an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids, which can then be further degraded through β-oxidation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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