Intermediate

Part:BBa_K398011

Designed by: Kira Schipper and Mathias Voges   Group: iGEM10_TU_Delft   (2010-10-07)

J23100_J61100_AlkB2_J61100_rubA3

AlkB2 encodes for a medium-chain alkane 1-monooxygenase originating from Gordonia sp. TF6. It is capable of converting C5-C13 alkanes along with C5-C8 cycloalkanes to the corresponding (cyclo)alkanols. It requires rubA3, rubA4 and rubB for proper functioning of the electron transfer system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None