Device

Part:BBa_K3978052

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


kill switch(arabinose)

This part is a kill switch induced by L-arabinose.In the absence of arabinose, the dimerization repressor protein AraCs will bind to the AraI1 operator site of PBAD and block the transcription of PBAD. When L-arabinose is added, AraC dimerization binds to it leading to conformational changes and thereby allowing downstream transcription of toxic protein MazF. This part consists of two parts, induced promoter(BBa_K2457000), toxic protein Mazf(BBa_K823044)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024


[edit]
Categories
Parameters
None