Plasmid

Part:BBa_K3978048

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


reget-plasmid-cIcIDN

This part is composed of six basic parts, estrogen response element(ERE) as a promoter, repression protein CI and a mutant CIDN tagged with ssrA tail, and two BRS with different effects. BBa_K3978043 is a weaker RBS used for CI and BBa_K3978044 is a stronger one for CIDN.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal XbaI site found at 5457
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 112
    Illegal PstI site found at 131
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 112
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal XbaI site found at 5457
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal XbaI site found at 5457
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1256
    Illegal SapI.rc site found at 2916


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Categories
Parameters
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