Plasmid

Part:BBa_K3978047

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


reget-plasmid-mazF

This part is composed of three basic parts.BBa_K3978013 is a plasmid backbone. BBa_R0051 is a promoter under the control of protein CI. BBa_K823044 is a toxic protein mazF. This part acts as a killer in the killing switch. When CI is expressed and combines with the promoter PRM, mazF will be expressed leading to cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 112
    Illegal PstI site found at 131
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 112
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1256
    Illegal SapI.rc site found at 2916


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Categories
Parameters
None