Coding

Part:BBa_K3978040

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


neget-ClpA

This part codes the protease ClpA. ClpA is an ATP-dependent protease, degrading proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. In the kill switch we designed, ClpA will degrade cIDN tagged by ssrA tails.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1915
    Illegal BglII site found at 1901
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1915
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1915
    Illegal NgoMIV site found at 1480
    Illegal AgeI site found at 310
    Illegal AgeI site found at 1458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None