Coding
Part:BBa_K3978040
Designed by: Minqi Hu Group: iGEM21_HiZJU-China (2021-10-21)
neget-ClpA
This part codes the protease ClpA. ClpA is an ATP-dependent protease, degrading proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. In the kill switch we designed, ClpA will degrade cIDN tagged by ssrA tails.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1915
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1915
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1915
Illegal BglII site found at 1901 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1915
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1915
Illegal NgoMIV site found at 1480
Illegal AgeI site found at 310
Illegal AgeI site found at 1458 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |