Coding

Part:BBa_K3978039

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


neget-ClpX

This part codes the protease ClpX. ClpX is an ATP-dependent protease, degrading proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. In the kill switch we designed, ClpX will degrade cIDN tagged by ssrA tails.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal BglII site found at 879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal AgeI site found at 361
    Illegal AgeI site found at 1099
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None