Coding

Part:BBa_K3978032

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


neget-cIDN-N55K

This part codes a mutant of CI repressor bearing a mutation in the DNA binding surface, Asn55Lys (N55K) that eliminated the binding affinity of the CI-mutant to operator sites but increased the affinity to non-specific DNA binding sites. It acts as a dominant negative inhibitor of the CI protein itself, presumably by forming mixed dimers as has been observed for the tet and lac repressors. Therefore, it can be used for blocking dimer formation of CI proteins, which will inactivate the promoter PRM of the kill switch.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 193
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 193
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 193
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None