Plasmid_Backbone

Part:BBa_K3978013

Designed by: Haozhen He   Group: iGEM21_HiZJU-China   (2021-10-21)


BL21 vector combined with amoA and hao gene sequence to degrade EE2 in wastewater

The pETDuet vector can combined both monoamine oxidase and hydroxylamine Oxidase coding gene. The petDuet vector use T7 promoter and lac promoter to express the protain also contains AmpR gene for selection.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 112
    Illegal PstI site found at 131
    Illegal NotI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 112
    Illegal BglII site found at 305
    Illegal BamHI site found at 106
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 112
    Illegal XbaI site found at 30
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1256
    Illegal SapI.rc site found at 2916


[edit]
Categories
Parameters
None