Plasmid
pGBKT7-ERa

Part:BBa_K3978004

Designed by: Ding Haote   Group: iGEM21_HiZJU-China   (2021-10-21)


It is a plasmid which can express a fusion protein of BD and ERa-421F.

It is a plasmid which can express a fusion protein of BD and ERa-421F. Coding sequence of target gene was inserted into an expression vectors with ERa-421F and Plasmid(BBa_K289008) to obtain BBa_K3978004. We transformed the constructed plasmid into yeast Y2H to construct yeast two-hybrid technology system.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5126
    Illegal XbaI site found at 6114
    Illegal XbaI site found at 7715
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 1321
    Illegal NotI site found at 1326
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1299
    Illegal BglII site found at 7598
    Illegal BamHI site found at 1309
    Illegal BamHI site found at 8081
    Illegal XhoI site found at 979
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5126
    Illegal XbaI site found at 6114
    Illegal XbaI site found at 7715
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5126
    Illegal XbaI site found at 6114
    Illegal XbaI site found at 7715
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 3099
    Illegal NgoMIV site found at 3383
    Illegal NgoMIV site found at 7133
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal BsaI site found at 898
    Illegal BsaI site found at 2887
    Illegal SapI site found at 1841
    Illegal SapI site found at 3323
    Illegal SapI site found at 3533


[edit]
Categories
Parameters
None