Coding

Part:BBa_K3978001

Designed by: Ding Haote   Group: iGEM21_HiZJU-China   (2021-10-21)


GRIP 1, a protein which can bind to estrogen receptor（ER）

The bait plasmid PGBKT7-ERα-LBD and the hunting plasmid PGADT7-Grip1 were constructed from the ligand binding domain of nER and glutamate receptor interacting protein（GRIP 1）, respectively. When EE2 exits, the complex formed by EE2-ERα and GRIP binds to the T7 promoter to initiate the expression of report genes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1626
    Illegal XbaI site found at 2682
    Illegal XbaI site found at 3051
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 276
    Illegal BglII site found at 2361
    Illegal BglII site found at 2406
    Illegal BglII site found at 2454
    Illegal BglII site found at 2637
    Illegal BglII site found at 2673
    Illegal BglII site found at 2709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1626
    Illegal XbaI site found at 2682
    Illegal XbaI site found at 3051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1626
    Illegal XbaI site found at 2682
    Illegal XbaI site found at 3051
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2521


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