Plasmid_Backbone

Part:BBa_K3978000

Designed by: Ding Haote   Group: iGEM21_HiZJU-China   (2021-10-21)


Yeast two-hybrid "prey" vector for expressing proteins fused to the GAL4 activation domain.

The pGADT7 vector expresses proteins fused to amino acids of the GAL4 activation domain (AD). In yeast, fusion proteins are expressed at high levels from the constitutive ADH1; transcription is terminated by the ADH1 transcription termination signals. The pGADT7 vector also contains T7 promoter, SV40 NLS and Kozak sequence. The vector carries the Amp+ for selection in yeast. Yeast strains containing pGADT7 exhibit a higher transformation efficiency than strains carrying other AD protein vectors. The pGADT7 vector is always used in yeast two-hybrid technology for detection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal NheI site found at 4496
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
    Illegal NotI site found at 4517
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal BglII site found at 1898
    Illegal BamHI site found at 2018
    Illegal XhoI site found at 2030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
    Illegal NgoMIV site found at 210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 860
    Illegal BsaI.rc site found at 5714
    Illegal SapI site found at 4631


[edit]
Categories
Parameters
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