Coding

Part:BBa_K3973000

Designed by: Sreenivas Eadara   Group: iGEM21_Hopkins   (2021-10-20)


UPC2 transcriptional regulator

UPC2 is a transcriptional regulator that is regulated by ergosterol binding. UPC2 will bind to Sterol Regulatory Elements (SREs) reversibly, where ergosterol binding discourages SRE binding.

UPC2, along with a promoter containing an SRE, can form a biosensor for fungi. Ergosterol is a molecule unique to fungal membranes and ergosterol concentration can be an indicator of fungal population. The 2021 Hopkins iGEM team used UPC2 for this purpose to regulate the production of antifungal genes in response to A. sydowii infection of corals.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 664
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 204
    Illegal AgeI site found at 1120
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None