Portion of the Neutral Site 3 of the Synechococcus elongatus UTEX 2973 genome.
This part is a portion of the Neutral Site 3 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 3 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid . This was one of the homology arms to Neutral Site 3 in our composite part BBa_K3971028 and the composite part BBa_K3971030 which were designed to insert sps and spp genes in the Neutral Site 3 of the S. elongatus genome respectively, under different promoters.
1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776
Sequence and Features
- 10Illegal PstI site found at 176
- 12Illegal PstI site found at 176
- 21COMPATIBLE WITH RFC
- 23Illegal PstI site found at 176
- 25Illegal PstI site found at 176
- 1000COMPATIBLE WITH RFC