Promoter characterization cassette for native E.coli csc operon bidirectional promoter.
This composite part has been designed to characterize the activity of the native E.coli csc operon bidirectional promoter from the strain EC3132 . In order to allow for modularity in the promoter region of the cassette, two restriction sites Nde1 and BamH1 flank it, so that the promoter can be easily excised out and replaced with another and the cassette can be used to characterize that promoter.
A YFP and a BFP gene flank the promoter on both sides on opposite coding strands, and the relative fluorescence intensity can be used as a proxy for the strength of the promoter in both directions. Both fluorescence promoters have a double terminator (BBa_B0015).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1699
 Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., & Lengeler, J. W. (2002). Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132. Journal of bacteriology, 184(19), 5307–5316. https://doi.org/10.1128/JB.184.19.5307-5316.2002