Other

Part:BBa_K3963004

Designed by: Silja Malkewitz   Group: iGEM21_Heidelberg   (2021-10-20)


pBAV1K origin of replication

pBAV1k origin of replication (ori) which is based on the pWV01 ori. The sequence was optimized for high copy number [1]. The ori was successfully tested in Escherichia coli DH5ɑ and Acinetobacter baylyi ADP1.

References

[1] Bryksin, A. V., & Matsumura, I. (2010). Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. PloS one, 5(10), e13244. https://doi.org/10.1371/journal.pone.0013244

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None