Other
Part:BBa_K3963004
Designed by: Silja Malkewitz Group: iGEM21_Heidelberg (2021-10-20)
pBAV1K origin of replication
pBAV1k origin of replication (ori) which is based on the pWV01 ori. The sequence was optimized for high copy number [1]. The ori was successfully tested in Escherichia coli DH5ɑ and Acinetobacter baylyi ADP1.
References
[1] Bryksin, A. V., & Matsumura, I. (2010). Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. PloS one, 5(10), e13244. https://doi.org/10.1371/journal.pone.0013244
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |