Generator

Part:BBa_K3962355

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-13)


Constant expression of antitoxin CcdA by constitutive promoter J23117

This construct is composed of constitutive promoter BBa_J23117 (from the Anderson promoter collection) and antitoxin CcdA of CcdA/CcdB type II originating from E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None