
Generator
Part:BBa_K3962352
Designed by: Siheng Li Group: iGEM21_Leiden (2021-10-13)
Inducible expression of antitoxin CcdA by pBAD
Description:This construct is composed of L-arabinose mediated inducible promoter pBAD and antitoxin CcdA of CcdB/CcdA toxin-antitoxin system. CcdB/CcdA is a type II toxin-antitoxin system originating from E. coli. This construct can be used to characterize the antagonistic effect of CcdA when combining with CcdB expression constructs. It can also be used as a biocontainment component, as when bacteria escape to the natural environment without the inducers the antitoxin gene is repressed.
Sequence and Features
Subparts|Ruler|SS|DSScars: Show|HideVertical: Show|HideLength: UnknownGet part sequenceView plasmid

Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
None |