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Generator
Part:BBa_K3962348
Designed by: Siheng Li Group: iGEM21_Leiden (2021-10-12)
J23113 promoter regulated constant expression of toxin RelE
This construct is composed of constitutive promoter J23113 (from the Anderson promoter collection) and toxin RelE. RelE/RelB is a type II toxin-antitoxin system originating from the E. coli chromosome. In our project, we used this construct to create a balanced RelE/RelB toxin-antitoxin system for preventing horizontal gene transfer.
Sequence and Features
Subparts|Ruler|SS|DSScars: Show|HideVertical: Show|HideLength: UnknownGet part sequenceView plasmid
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Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |