arabinose-mediated inducible expression of toxin RelE
This construct is composed of L-arabinose mediated inducible promoter pBAD and toxin RelE. RelE/RelB is a type II toxin-antitoxin system originating from E. coli. In our project, we used this construct to test the toxicity of toxin RelE, which provided us with more insights in regulating the balance of the toxin-antitoxin system for preventing horizontal gene transfer.
2021 Team Leiden
RelE has been used as a part of a kill switch. The lethality of this kill switch has been tested in a plate reader assay (Fig. 1)
Figure 1. Plate reader assay of E. coli TOP 10 cellular growth over time, with a gradient of arabinose concentrations.
Cell growth is not inhibited following the induction of RelE. The results show that RelE is not enough to kill cells immediately when grown in liquid LB medium.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000Illegal SapI site found at 961