Part:BBa_K3956010

scl2

Scl2, or streptococcal collagen-like protein, is a bacterial collagen-like protein used by S. pyogenes in biofilm adhesion. It has been thought to closely resembles Type 1a human collagen in terms of potential applications.

Usage and Biology

Scl, streptococcal collagen-like protein, is a bacterial collagen that has been well-documented in recent years. The key distinction between bacterial and mammalian collagen is the hydroxyproline post-translational mechanisms: mammalian collagen requires this complex process whereas bacterial collagen does not. Past studies show that Scl2 has similar enough properties to human collagen to be used as biomaterials while also avoiding the lengthy processes of hydroxylation.

Going deeper into Scl2, most types of scl proteins, including Scl2, are naturally expressed in the human pathogen, Streptococcus pyogenes, also known as Group A streptococcal (GAS). The Scl proteins are virulence factors of S. pyogenes and contribute to the formation of biofilm adhesion and infection of different species (Squeglia et al 14). The Scl2 gene itself is made of several parts: the V domain, the triple helical CL domain, and the tail. When building the plasmid to express Scl2, we had to add a 5’UTR in front of the codon to make sure the triple helical structure was produced.

The enzyme cleavage site has been synthetically added in order to isolate the CL domain. In addition, a His tag was added for purification and visualization. Compared to scl1, scl2 has a highest melting point and other thermal denaturation properties that make it more useful for our project. Additionally, scl2 is easier to synthesize and for those that aim to build on our project, using scl2 will be much more convenient. Additionally, scl2 is the preferred bacterial collagen for our project because it has better structural integrity with a longer protein expression compared to scl1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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