Part:BBa_K3956008
scl2 CL domain (collagen-like)
The scl2 CL domain is the desired protein domain that resembles Type 1a collagen. In order to extract it, the whole protein, including the globular domain, should be expressed and cleaved off using an enzyme binding site and a linking sequence.
Usage and Biology
Scl2, a streptococcal collagen-like protein, is a bacterial collagen that has been well-documented in recent years. The key distinction between bacterial and mammalian collagen is the hydroxyproline post-translational mechanisms: mammalian collagen requires this complex process whereas bacterial collagen does not. Past studies show that Scl2 has similar enough properties to human collagen to be used as biomaterials while also avoiding the lengthy processes of hydroxylation.
Going deeper into Scl2, most types of scl proteins, including Scl2, are naturally expressed in the human pathogen, Streptococcus pyogenes, also known as Group A streptococcal (GAS). The Scl2 proteins are virulence factors of S. pyogenes and contribute to the formation of biofilm adhesion and infection of different species (Squeglia et al 14). The Scl2 gene itself is made of several parts: the V domain, the triple helical CL domain, and the tail.
In CCA_San_Diego's project, the CL domain was added after the V domain connected by an enzyme cut site and bridging sequence, and preceded a PRM9 terminator as this sequence is codon-optimized for yeast.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 154
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