Part:BBa_K3956007
scl2_Triple helix bridging sequence
The bridge sequence goes between the enzyme cut site and the collagen-like domain to ensure there is structural support when the protein is cleaved to separate the CL domain.
Usage and Biology
This part was used in CCA_San_Diego’s 2021 CollaGene project after the globular domain of collagen and an enzyme cut sit and directly before the Collagen-Like domain with Gly-Xaa-Yaa repeats. Although we were able to verify that the construct had been correctly inserted into appropriate selective plates by colony PCR verification, the protein expression was not able to be verified within the time constraints of the competition.
We want to emphasize that this sequence may still be successful, but its efficiency was unable to be demonstrated within the time span that CCA_San_Diego had in the lab. For future teams, we advise testing construct designs with successful expression with and without the sequence to compare expression levels.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |