Part:BBa_K3955000

Curli assembly protein CsgC

csgC is a chaperone that is part of the curli production system. It acts as a chaperone to prevent aggregation of csgA in the periplasm. It has also been shown to prevent curli fibril elongation from csgA in vitro [1, 2], and we had expected to use it for curli inhibition. However, while we successfully inserted it into pET-11a, we did not successfully express it.

References

1. Evans M, Chorell E, Taylor J, Åden J, Götheson A, Li F et al. The Bacterial Curli System Possesses a Potent and Selective Inhibitor of Amyloid Formation. Molecular Cell. 2015;57(3):445-455.

2. Taylor J, Hawthorne W, Lo J, Dear A, Jain N, Meisl G et al. Electrostatically-guided inhibition of Curli amyloid nucleation by the CsgC-like family of chaperones. Scientific Reports. 2016;6(1).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]