Reporter
PompC(C)

Part:BBa_K395304

Designed by: THIPRAMPAI THAMAMONGOOD   Group: iGEM10_Tokyo_Tech   (2010-10-02)

PompC(C) with GFP reporter (K395301+E0240)

This part is contructed for measuring the transcriptional activity of OmpC(C) promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 24
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


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Categories
Parameters
None