Part:BBa_K3947004

CcVHPO3 Vanadium-Dependent Haloperoxidase

This gene was retrieved from Chondrus Crispus (Thapa et al.,2020). This gene is analogous to the Asparagopsis taxiformis bromoperoxidase. They follow the same principle and mechanism as the A.Taxiformis enzymes. It is an enzyme from the family of bromide peroxidases and catalyzes the following reaction: HBr + H₂O₂ → HOBr + H₂O

The enzyme uses Vanadium as a co-factor in the catalysis of the bromoform. The active site features a vanadium oxide center attached to the protein via one histidine side chain. The enzymes catalyze the oxidation of bromide by hydrogen peroxide. Bromonium cation (Br⁺) that is generated attacks hydrocarbons (represented as R). They are only specific to the bromide and are unable to oxidize chloride at all. R-H + Br^- + H₂O₂ -> R-Br + H₂O₂ + OH^-

Sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 515
  Illegal BamHI site found at 623
  Illegal BamHI site found at 639
  Illegal BamHI site found at 1131
  Illegal BamHI site found at 1793
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 128
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Thapa, H. R., Lin, Z., Yi, D., Smith, J. E., Schmidt, E. W., & Agarwal, V. (2020). Genetic and biochemical reconstitution of bromoform biosynthesis in asparagopsis lends insights into seaweed reactive oxygen species enzymology. ACS Chemical Biology, 15(6), 1662–1670. https://doi.org/10.1021/acschembio.0c00299