Part:BBa_K3947002

Mbb4 - Vanadium-Dependent Haloperoxidase

Gene sequence that was identified in the seaweed Asparagopsis taxiformis by (Thapa et al., 2020). It is an enzyme from the family of bromide peroxidases and catalyzes the following reaction: HBr + H₂O₂ → HOBr + H₂O

The enzyme uses Vanadium as a co-factor in the catalysis of the bromoform. The active site features a vanadium oxide center attached to the protein via one histidine side chain. The enzymes catalyze the oxidation of bromide by hydrogen peroxide. Bromonium cation (Br⁺) that is generated attacks hydrocarbons (represented as R). They are only specific to the bromide and are unable to oxidize chloride at all. R-H + Br^- + H₂O₂ -> R-Br + H₂O₂ + OH^-

Sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 264
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 346
  Illegal NgoMIV site found at 844
  Illegal AgeI site found at 1221
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Thapa, H. R., Lin, Z., Yi, D., Smith, J. E., Schmidt, E. W., & Agarwal, V. (2020). Genetic and biochemical reconstitution of bromoform biosynthesis in asparagopsis lends insights into seaweed reactive oxygen species enzymology. ACS Chemical Biology, 15(6), 1662–1670. https://doi.org/10.1021/acschembio.0c00299