Part:BBa_K3947001

Mbb2 - Reactive Oxygen Species Enzyme

They are membrane bound enzymes and catalyze the transfer of the electrons from the electron donor, NAD(P)H, via flavin and heme cofactors to molecular oxygen generating a Reactive Oxygen Species – hydrogen peroxide and superoxide anion.

A family of enzymes that are generators of reactive oxygen species, which are called nicotinamide adenine dinucleotide phosphate (NADPH) oxidase family of enzymes (NOXs) for short. They are membrane bound enzymes and catalyze the transfer of the electrons from the electron donor, NAD(P)H, via flavin and heme cofactors to molecular oxygen generating a reactive Oxygen Species – hydrogen peroxide and superoxide anion.

Mbb2 enzyme is distinct from the other NOXs due to the positioning of the membrane-spanning heme-binding/oxygen reducing domain as well as the flavin-binding/NAD(P)H-oxidizing cytoplasmic domain.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1154
  Illegal BamHI site found at 2124
  Illegal XhoI site found at 837
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 448
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Thapa, H. R., Lin, Z., Yi, D., Smith, J. E., Schmidt, E. W., & Agarwal, V. (2020). Genetic and biochemical reconstitution of bromoform biosynthesis in asparagopsis lends insights into seaweed reactive oxygen species enzymology. ACS Chemical Biology, 15(6), 1662–1670. https://doi.org/10.1021/acschembio.0c00299