Coding

Part:BBa_K3939008

Designed by: Haiyang Liu   Group: iGEM21_Tianjin   (2021-09-30)


tetR gene expression product

Insert the tetO sequence into the proper position of the promoter. When there is tetR expression product, it can prevent the promoter from turning on the expression of downstream genes.When induced by adding anhydrous tetracycline, it can prevent tetR from binding to tetO and turn on downstream gene transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 122
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None