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Part:BBa_K3933020

Designed by: Julian Leduc   Group: iGEM21_Ionis_Paris   (2021-10-21)


Golden Gate Esp3I cloning site

This part was designed to be inserted into a RFC[10] compatible backbone. Its sequence contains two Esp3I/BsmBI restriction enzyme sites for Golden Gate Assembly.

Golden Gate overhang sequences need to be designed as followed : 5' CCGCN 3' at 5' of the insert 5' NAACT 3' at 3' of the insert


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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