Lactate dehydrogenase regulated by a Fluoride riboswitch
This part is a composite part composed of a constitutive promoter, a fluoride riboswitch, a RBS, a lactate dehydrogenase and a terminator. It is an adaptation of the Cobatect project for other purposes than detecting vitamin B12 but it could not be tested in the lab.
More details can be found on (BBa_K911003) , but we tried to sum up some information found on this page here :
It is a positive regulator with a high sensitivity to the ion F-. In the absence of fluoride, the riboswitch, which was originally identified from Bacillus cereus, acts as a transcriptional attenuator. Its structure changes in the presence of fluoride, and the repression is released. This allows B. cereus to translate a fluoride efflux pump, which allows the bacteria to cope with potentially dangerous fluoride levels in its environment. It's categorized into three types of chassis: TOP10 e.coli, bacillus subtilis laboratory strain, and bacillus subtilis with the usual fluoride riboswitch gene (the crcB gene) knocked out.
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Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 26
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 639
- 1000Illegal SapI.rc site found at 369