Composite

Part:BBa_K3933006

Designed by: Marie Guillaume   Group: iGEM21_Ionis_Paris   (2021-10-19)


Lactate dehydrogenase induced by arabinose

This part is a composite part constituted of a Lactate Dehydrogenase (BBa_K3102006) , framed by an arabinose promoter (BBa_I0500) and a double terminator (BBa_B0015) .

Lactate Dehydrogenase is an enzyme catalyzing the interconversion of pyruvate into lactate. We added an arabinose promoter to be able to control the production of the enzyme and thus control the quantity of lactate produced depending on the quantity of arabinose added to the system.


BBa K3933006 schema.png

Thanks to Biorender


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1406
    Illegal SapI site found at 961


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Categories
Parameters
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