Part:BBa_K3933003
Vitamin B12 Riboswitch
This part is a Vitamin B12 Riboswitch, which acts as a sequester of the RBS located downstream of the RNA sequence of the riboswitch. This riboswitch inhibits the translation of the coding sequence placed after, by hindering the binding of the ribosome to the RNA sequence. This riboswitch was found in Propinobacterium shermanii, upstream of the gene cbiB, which is a Cobalamin biosynthesis gene. It has been demonstrated that it is sensible to Cobalamin. [1]
When searching for a riboswitch sequence to use for our project, we found this sequence which was previously used by team Paris Bettencourt 2015 and team OUC China 2019 (BBa_K2904100) . However, this part was not referenced alone as a basic part, but already combined with a diverse components such as a lac promoter and an mRFP. Paris Bettencourt used the riboswitch as a Cobalamin biosensor, by coupling it directly first with a GFP, then with an mRFP. As their results were not optimal, team OUC China decided to add a stabilizer and a tuner to their construction to have better results. All these information can be found on the BBa_K2904100 page.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Li, J., Ge, Y., Zadeh, M., Curtiss, R., & Mohamadzadeh, M. (2020). Regulating vitamin B12 biosynthesis via the cbiMCbl riboswitch in Propionibacterium strain UF1. Proceedings of the National Academy of Sciences of the United States of America, 117(1), 602–609. [1](https://doi.org/10.1073/pnas.1916576116)
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