Generator

Part:BBa_K393000

Designed by: Jon Russel, Leah Itagaki   Group: iGEM10_Yale   (2010-10-25)


phsABC_generator

This part contains a ribosome binding site the phsABC gene from Salmonella enterica serovar Typhimurium and a transcription terminator. The phsABC gene is a thiosulfate reductase that causes production of hydrogen sulfide gas. Bang et al. (AEM 2000) expressed this gene in E. coli to precipitate Cadmium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2069
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 121
    Illegal AgeI site found at 2644
    Illegal AgeI site found at 3427
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None