Composite

Part:BBa_K3921007

Designed by: Kai Liu   Group: iGEM21_XHD-Wuhan-B-China   (2021-10-21)


araC/pBAD-dicF-T1/T2

In order to verify that sRNA DicF can indeed inhibit the cell division of MG1655, we construct this circuit.



When induced by arabinose, DicF was expressed and MG1655 cell division was inhibited. See BBa_K3921006 for detailed experimental results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None