Composite

Part:BBa_K392046

Designed by: Teoh Shao Thing, Kousuke Torigata, Rie Takino   Group: iGEM10_Osaka   (2010-11-07)

endoglucanase expression device

Device consisting of C. fermi endoglucanase CenA with promoter, ribosome binding site, terminator. Expresses enough endoglucanase to degrade filter paper.

See experience page for experimental data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 260
    Illegal NotI site found at 1404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1317
    Illegal BamHI site found at 453
    Illegal XhoI site found at 815
    Illegal XhoI site found at 1064
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 595
    Illegal NgoMIV site found at 1520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 499


[edit]
Categories
Parameters
None