Coding

Part:BBa_K392032

Designed by: Teoh Shao Thing, Rie Takino, Kosuke Torigata   Group: iGEM10_Osaka   (2010-10-25)


yeast secretion tag + C. fermi beta-glucosidase

C. fermi beta-glucosidase fused with engineered yeast secretion tag for extracellular expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1196
    Illegal XhoI site found at 1429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 685
    Illegal AgeI site found at 128
    Illegal AgeI site found at 187
    Illegal AgeI site found at 581
    Illegal AgeI site found at 776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 558


[edit]
Categories
Parameters
None