Coding

Part:BBa_K392031

Designed by: Teoh Shao Thing, Rie Takino, Kosuke Torigata   Group: iGEM10_Osaka   (2010-10-25)


yeast secretion tag + C. fermi exoglucanase

C. fermi enxoglucanase Cex fused with engineered yeast secretion tag for extracellular expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 643
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 276
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696
    Illegal SapI.rc site found at 779


[edit]
Categories
Parameters
None