Protein_Domain

Part:BBa_K392006

Designed by: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda   Group: iGEM10_Osaka   (2010-10-24)

C. firmi CenA (Silver standard compatible)

Endoglucanase from C. firmi re-cloned into Silver standard-compatible part.

See experience page for experimental data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 31
    Illegal NotI site found at 1175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1088
    Illegal BamHI site found at 224
    Illegal XhoI site found at 586
    Illegal XhoI site found at 835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 366
    Illegal NgoMIV site found at 1291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 270


[edit]
Categories
Parameters
n/aC. firmi CenA (Silver standard compatible)