Composite

Part:BBa_K391008

Designed by: Marianne (Minju) Park   Group: iGEM10_British_Columbia   (2010-10-27)


constitutive promoter-RBS-DspB-terminator (J23100:B0034:K391006:B0014)

Strongest constitutive promoter in J23100 family (J23100) + strongest RBS in the community collection (B0034)+ Dispersin B carbohydrate digesting enzyme (K391006) + double terminator (B0014).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1030
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None