Primer

Part:BBa_K391004

Designed by: Phillip Chu   Group: iGEM10_British_Columbia   (2010-10-25)


Forward primer to PCR S. aureus plasmid replicon

S. aureus plasmid replicon. Can allow a BioBrick plasmid to replicate and segregate in S. aureus. Derived from pT181. Can use either plasmids pT181 or plasmids derived from pT181 (e.g. pcN33). Add EcoRI and SpeI sites before using. Expect a band ~2kbp after PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None